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Nano Bio Optics Lab (Yoav Shechtman)
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Nano Bio Optics Lab (Yoav Shechtman)

@ShechtmanLab

We develop microscopy methods to observe life on the nanoscale

292 Following1K Followers249 Posts
  2. Ricardo Henriques@HenriquesLab
    If robots could dream of microtubules, how would they look like? An amazing story by Alon Saguy, @ShechtmanLab and colleagues. Proud we could contribute to it
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  5. Nano Bio Optics Lab (Yoav Shechtman)@ShechtmanLab
    PSF engineering typically requires a 4-f system, but this is not always feasible, e.g. due to size constraints. Nadav Opatovski attached a phase mask directly to the objective lens, see beads defocusing here. Tetrapod mask shown here (wait for the end):
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  6. Nano Bio Optics Lab (Yoav Shechtman)@ShechtmanLab
    Posts @ShechtmanLab
    This enables PSF engineering in space-limited microscopy systems, e.g. that fit into incubators. Uses include extended depth-of-field and snapshot 3D spheroid from 2D, using algorithms by @EliasNehme9. Bonus: Obtaining training images is easy with a high-throughput microscope.
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  8. Nano Bio Optics Lab (Yoav Shechtman)@ShechtmanLab
    🥳 DBlink is now peer reviewed and improved! Online in @naturemethods: Congrats @AlonSaguy, @AlaloufOnit, @HeilemannLab et al.!
    Nano Bio Optics Lab (Yoav Shechtman) @ShechtmanLab
    New prepint just out! Live cell single molecule localization microscopy at >3 fps. The information is mostly there - just spread out in space and time :) We use a recurrent neural net (LSTM) + some assumptions to extract it. All details here:
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  9. Nano Bio Optics Lab (Yoav Shechtman)@ShechtmanLab
    New prepint just out! Live cell single molecule localization microscopy at >3 fps. The information is mostly there - just spread out in space and time :) We use a recurrent neural net (LSTM) + some assumptions to extract it. All details here:
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  10. Nano Bio Optics Lab (Yoav Shechtman)@ShechtmanLab
    One of these super-resolution microtubule images was captured by a microscope and the other one was generated by a diffusion model. Can you tell which is which? Try more here: 1/4
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  11. Nano Bio Optics Lab (Yoav Shechtman)@ShechtmanLab
    Posts @ShechtmanLab
    Alon Saguy et al. trained a diffusion model to generate synthetic images of super-resolution microscopy images using data of only a few images from ShareLoc ( 2/4
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  13. Nano Bio Optics Lab (Yoav Shechtman)@ShechtmanLab
    Posts @ShechtmanLab
    This is a very ongoing project, with many open questions: what are the performance limits? How much can we really gain beyond the information in the training data? and of course: what would be the best uses of something like this in super-resolution microscopy? 4/4
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  17. Nano Bio Optics Lab (Yoav Shechtman)@ShechtmanLab
    Computational microscopy often relies on some kind of wavefront shaping by diffractive optical components, which can be very difficult to fabricate. This motivated us to utilize 3D printing + near index matching for simple & cheap DOE fabrication.
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  18. Nano Bio Optics Lab (Yoav Shechtman)@ShechtmanLab
    Posts @ShechtmanLab
    We generate a variety of solid elements, including microlenses, vortex plates for MINSTED and more, and pretty challenging PSF engineering masks! Thanks to our collaborating groups, Ady Arie, @Stefan_W_Hell @AleksPonjavic, in this multi-national effort led by Reut Kedem Orange!
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  20. Nano Bio Optics Lab (Yoav Shechtman)@ShechtmanLab
    In many (most?) cases in fluorescence microscopy, in which the positions of spots are determined, it is not really their global positions that are of interest, but rather their *relative* positions, i.e. their distances from one another.
    Intelligent Computing @IntellComput
    #IntelligentComputing New research article Learning Optimal Multicolor PSF Design for 3D Pairwise Distance Estimation Ofri Goldenberg, Boris Ferdman, Elias Nehme, Yael Shalev Ezra, and Yoav Shechtman @ShechtmanLab Special Issue "Computational Imaging"
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  21. Intelligent Computing@IntellComput
    #IntelligentComputing New research article Learning Optimal Multicolor PSF Design for 3D Pairwise Distance Estimation Ofri Goldenberg, Boris Ferdman, Elias Nehme, Yael Shalev Ezra, and Yoav Shechtman @ShechtmanLab Special Issue "Computational Imaging"
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  22. Nano Bio Optics Lab (Yoav Shechtman)@ShechtmanLab
    Posts @ShechtmanLab
    Consider the problem of determining the 3D distance between a pair of 2-colored spots. One option is to find x1,y1,z1 and x2,y2,z2, e.g. using a 3D PSF, and calculate the distance. But why not optically encode the *distance*, rather than individual positions?
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  23. Nano Bio Optics Lab (Yoav Shechtman)@ShechtmanLab
    Posts @ShechtmanLab
    Just out: Ofri Goldenberg learned and implemented a multicolor phase mask + decoding net for determining the distance between a pair of 2-colored spots in 3D. The net outputs the scalar distance of interest.
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  24. Nano Bio Optics Lab (Yoav Shechtman)@ShechtmanLab
    cool idea + implemented using simple elements!
    Dedecker Lab (dedeckerlab.bsky.social) @DedeckerLab
    Doing multicolor (single-molecule) imaging? Tired of waiting for each color? Want simultaneous imaging with full field of view but without the multicamera hassle? Take a look at our 'Circulator'! 🔥💪 Very excited so please RT! (1 of 5) #microscopy
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  25. Dedecker Lab (dedeckerlab.bsky.social)@DedeckerLab
    Doing multicolor (single-molecule) imaging? Tired of waiting for each color? Want simultaneous imaging with full field of view but without the multicamera hassle? Take a look at our 'Circulator'! 🔥💪 Very excited so please RT! (1 of 5) #microscopy
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    759220
  26. Nano Bio Optics Lab (Yoav Shechtman)@ShechtmanLab
    Can you determine your distance from a truck the same way you determine the z position of a fluorescent molecule? Of course you can, all you need is a PSF-engineered telescope! Adventurous Nadav Opatovski built one:
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  27. Nano Bio Optics Lab (Yoav Shechtman)@ShechtmanLab
    Posts @ShechtmanLab
    As @HenriquesLab noted in the last SMLMS regarding the blinking Eiffel tower - Maxwell's equations are the same regardless of scale... BTW the failure of the active rangefinder in the gif is due to scatter from dust from the truck; PSF engineering is much more robust to it.
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  28. Nano Bio Optics Lab (Yoav Shechtman)@ShechtmanLab
    Posts @ShechtmanLab
    Phase mask is a double-helix, and for image analysis we used @SjoerdStallinga's excellent cepstrum method or a neural net by Dafei Xiao. More info in the paper + realization how fortunate we are in microscopy to neglect atmospheric turbulence + an excuse for us to buy a drone.
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