#PathwayRefactoring

Our new study is out in @NatureBiotech, demonstrating that #CarbonNegative production of chemicals enabled by #SyntheticBiology is both feasible and scalable. Interdisciplinary effort with @leangching, @jewettlab_NU/@NUSynBio & @ORNL supported by @ENERGY

We started on this journey over a decade ago, but when we first introduced acetone biosynthesis enzymes into C. autoethanogenum only low levels (few mM) of acetone was produced & genetic tools were still in its infancy (link below for history on tool development)

10 years ago,engineering acetogenic clostridia was considered impossible. Today,we have new tools thanks to help from some friends & hard work by @LanzaTech’s SynBio team @LeangChing @JensenRasmus @LoganReadnour @MaryPavan @Shivani64265327 @JBehrendorff @Microbiology @sim0nky 1/n

To achieve commercially viable acetone and isopropanol production, we employed a three-pronged approach that comprised innovations in #PathwayRefactoring, #StrainOptimization & #ProcessDevelopment that integrated #HTWorkflows, #Modeling, #ScaleUp & #LCA